Research Journal of Pharmacy and Technology

Mohamed Zerein Fathima ¹, T. S. Shanmugarajan ¹, I. Somasundharam ¹, J. Anisha Ebens ¹, T. T. Neethu ¹

Affiliations
1 Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels University (VISTAS), Pallavaram-600117, Tamilnandu, IN

Abstract

Trichosanthes cucumerina linn is used as a traditional medicine for various diseases. In the present study was conducted to evaluate theanalgesic activity for Petroleum ether and Methanol extract of Trichosanthes cucumerinalinn and the activity was compared with diclofenac sodium as a standardand assessed using acetic acid induced abdominal writhing in mice.The methanolic extract exhibited significant value (P<0.001)analgesic activity as evidenced by increased the percentage of reduction in reaction time. The results thus support the Trichosanthes cucumerina linn used as ananalgesic agent. The plant showed no sign of toxicityup to the dose of 100 mg/kg in mice.

Keywords

Trichosanthes cucumerina linn, Analgesic Activity, Phytochemical Screening, Structural Elucidation, IR.

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Mohamed Zerein Fathima ¹, T. S. Shanmugarajan ¹, S. Satheesh Kumar ¹, B. V. Venkata Nagarjuna Yadav ¹

Affiliations
1 Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels University (VISTAS), Pallavaram-600117, Tamilnandu, IN

Abstract

Cancer is fundamentally a disease of disordered gene expression. Hepatocellular carcinoma is a well known and third most common cancer worldwide. The protein - ligand interaction plays a imporatant role in structural based drug designing for disease. Hinokitiol (HIOL) is also known as β-thujaplicin, is found in the heartwood of cupressaceous plants and naturally occurring tropolane derivative is a known biochemical target other than the fact that it induces apoptosis. The docking scores of the active constituents are compared against the standard drugs. The 3D structures of the constituents (Nilotinib, Sorafenib and Hinokitiol) are obtained from PubChem compound. The target for docking studies is selected as Proto-oncogene tyrosine-protein kinase ABL1 and Mitogen-activated protein kinase. Docking analysis is done by initially selecting the target for the disease and followed by obtaining the 3D structure of Proto-oncogene tyrosine-protein kinase ABL1 and Mitogen-activated protein kinase 14 (PDB ID:2HZI) and (PDB ID: 3LFF) respectively from protein data bank. The ABL1 and MAPK was docked with the above said drugs and based upon the lipin rule, rerank score and mol dock score we chosen the hinokitiol having good interaction with receptor molecule and docking studies has been carried out through Molegro Virtual Docker (MVD).

Keywords

Hinokitiol, ABL1, MAPK, Hepatocellular Carcinoma, Lipin Rule.

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